Radioimmunoassay of serum ferritin.

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Radioimmunoassay of serum ferritin.

Purified human spleen ferritin was labelled with 125I. On Sepharose 6-B gel filtration four species of labelled products were separated: a component with a higher molecular weight than ferritin; a component which is eluted in the same volume as unlabelled ferritin; and two labelled compounds with molecular weights lower than ferritin. When these labelled materials were used in a double antibody...

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Implications of using different tissue ferritins as antigens for ferritin in serum: four radioimmunoassay kits compared.

We compared three serum ferritin radioimmunoassay kits and one noncommercial RIA for ferritin quantitation, with regression analysis of results for sera from 35 ostensibly healthy subjects. There was a good correlation (p less than 0.001) between these various RIAs, but the slope of the regression line varied widely, most probably because of lack of standardization of the serum ferritin assay. ...

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Serum ferritin.

(1) Brief introduction to iron metabolism and the biochemistry of ferritin. (2) Early studies of circulating ferritin. (3) Methods for measuring serum ferritin concentrations -- immunoradiometric, radioimmuno- and enzyme-linked immuno assays based on liver or spleen ferritin -- an evaluation of these techniques. (4) Serum ferritin concentrations in normal subjects -- definition of normality -- ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1978

ISSN: 0021-9746

DOI: 10.1136/jcp.31.9.872